Murine C2C12 cells: GTP-stimulated cells vs. Control in SM

To define the signalling of extracellular GTP as enhancer of myogenesis, we investigated if the gene expression profile of differentiated C2C12 cells (4 and 24 hours in SM) was affected by extracellular GTP. Two-condition experiment, GTP-treated C2C12 cells vs Control C2C12 cells at 4 h and 24 h of differentiation. Biological replicates: 1 control, 1 GTP-treated cells, independently grown and harvested. One replicate per array.

为明确细胞外三磷酸鸟苷（GTP）作为肌发生增强因子的信号调控通路，本研究探究了细胞外GTP对在骨骼肌分化培养基（SM）中培养4小时与24小时的分化型C2C12细胞的基因表达谱是否存在影响。本实验为双条件对照实验，在肌分化的4小时及24小时两个时间点，分别设置GTP处理组C2C12细胞与对照组C2C12细胞。生物学重复设置如下：对照组与GTP处理组各1份独立培养并收获的细胞样品，每个基因芯片（array）对应1个重复样本。