Organic matter and bacteria measurements of POLARSTERN cruise PS114 and Maria S. Merian cruise MSM77

The discrete biogeochemical measurements from RV Polarstern PS114 and RV Maria S. Merian MSM77 sampled for dissolved organic matter, heterotrophic bacteria and gel particles. The parameters include chlorophyll, DOC/TDN/TDP/DOP, dissolved hydrolysable amino acids, dissolved combined carbohydrates, heterotrophic bacterial abundance and production, gel particles like TEP and CSP. The samples were aquired within 0-100 m of the Greenland Sea between 78°N to 80°N and 2°W to 14°E. The data was collected daily on board PS114 from 16th July 2018-23rd July 2018 and on board MSM77 from 16th September 2018-4th October 2018. The water collection occured using a CTD and laboratory methods varied by parameter. Chlorophyll was extracted using acetone, DOC/TDN was analysed using High-Temperature Catalytic Oxidation (TOC-VCSH), TDP/DOP was analysed colorimetrically using Acidic Molybdate Solution, dissolved hydrolysable amino acids were analysed using High Performal Liquid Chromatography (HPLC) Ortho-phthaldialdehyde Derivatization, dissolved combined carbohydrates were analysed using High Performance Anion Exchange Chromatography (HPAEC) coupled with Pulsed Amperometric Detection (PAD), cell abundance was analysed using flow cytometery, bacterial production was analysed using radioactively labelled 3H-Leucine and apllication of the microcentrifuge method, gel particles were analysed microscopically followed by image analysis. The purpose of data collection was to understand seasonal cycling of organic mater and heterotrophic bacteria dynamics within microbial loop.

极地号科考船（RV Polarstern PS114）与玛丽亚·S·梅里安号科考船（RV Maria S. Merian MSM77）针对溶解有机物、异养细菌及凝胶颗粒开展的离散生物地球化学测量数据。测量参数包括叶绿素、溶解有机碳/总溶解氮/总溶解磷/溶解有机磷（DOC/TDN/TDP/DOP）、溶解水解氨基酸、溶解结合碳水化合物、异养细菌丰度与生产力，以及透明胞外聚合颗粒物（TEP）、胶体悬浮颗粒物（CSP）等凝胶颗粒。样本采集于格陵兰海78°N至80°N、2°W至14°E海域的0-100米水层。PS114船的数据于2018年7月16日至23日在船上每日采集，MSM77船的数据则于2018年9月16日至10月4日采集。水样通过温盐深仪（CTD）获取，各参数的实验室分析方法如下：叶绿素采用丙酮萃取法分析；DOC/TDN采用高温催化氧化法（TOC-VCSH）分析；TDP/DOP采用酸性钼酸盐溶液比色法分析；溶解水解氨基酸采用高效液相色谱（HPLC）邻苯二甲醛衍生化法分析；溶解结合碳水化合物采用高效阴离子交换色谱（HPAEC）联用脉冲安培检测（PAD）法分析；细胞丰度通过流式细胞术测定；细菌生产力采用放射性标记3H-亮氨酸结合微量离心法分析；凝胶颗粒通过显微镜观察结合图像分析测定。数据采集的目的是理解微生物环内有机物的季节循环及异养细菌动态。