Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool19_h1930002_A44-A45. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool19_h1930002_A44-A45

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞架构，对于理解其多样功能与复杂的动物行为至关重要。本研究对多个人类大脑脑区应用单细胞核甲基化测序（single nucleus methylation sequencing），鉴定得到多组独特的细胞簇，并以此构建了层级分类体系。这些细胞簇涵盖了已知的主要脑细胞类型以及潜在的细胞亚型。我们依托本次测序获得的数据，鉴定各细胞簇的表观基因组特征，并明确其特异性调控元件。
整体实验设计：采用snmC-seq3技术对脑区进行测序，以解析成年人类大脑的表观基因组图谱。