Metagomic study of Epinephelus fuscoguttatus fish gut under different breeding conditions. fish gut metagenome

We extracted and sequenced the total DNA out of faecal samples, orginating from Epinephelus fuscguttatus, an important Indonesian food fish species. The samples, derived under free-living and mariculture conditions, were processed by an own developed pipeline, based on blastn, to achieve taxonomical annotations for the bacterial communites and eukaryotic content and in addition,we provided a functional annotation to the sequence reads, based on Pfam Protein families. The results were analysed for their differences, regarding the two different breeding conditions, and the elucidation of eukaryotic content and bacterial communities in general.

本研究从印度尼西亚重要食用鱼类点带石斑鱼（Epinephelus fuscguttatus）的粪便样品中提取并测序了总DNA。采集自野生环境与海水养殖环境的该类样品，经基于BLASTn的自主开发分析流程处理，完成了细菌群落与真核生物组分的分类学注释；此外，本研究还基于Pfam蛋白质家族（Pfam Protein families）对序列读段进行了功能注释。本研究针对两种养殖环境下的组学特征差异，以及整体的真核生物组分与细菌群落结构开展了解析与差异分析工作。