Table6_SNX20AR/MiRNA-301a-3p/SNX20 Axis Associated With Cell Proliferation and Immune Infiltration in Lung Adenocarcinoma.xlsx

Lung cancer is the most common tumor with severe morbidity and high mortality. Increasing evidence has demonstrated that SNX20 plays crucial roles in the progression of human cancer. However, the functions and mechanism of SNX20 in LUAD are still barely known. Here, we employ the TCGA, GEO and CCLE databases to examine the expression of SNX20 in human varies cancer, the results shown that SNX20 is down-regulated in lung Adenocarcinoma, SNX20 level was significantly positive correlated with poor prognosis and lung cancer immune cell infiltration. We found that over-expression of SNX20 significantly restrain NSCLC cell proliferation and migration. Subsequently, we discover a network regulating SNX20 in LUAD, further study found that the decreased of the SNX20 likely caused by DNA hypermethylation. Furthermore, we identified that SNX20AR/miRNA-301a-3p mediated decreased of SNX20 correlated with lung cancer progression and cancer immune infiltration in LUAD. Our findings suggested that ncRNAs play a crucial role in the regulatory network of SNX20. Collectively, our findings demonstrate the suppressor roles of the SNX20AR/miRNA-301a-3p/SNX20 axis in Lung Adenocarcinoma, represent that SNX20 have the potential of as an effective therapeutic target in future.

肺癌是临床最常见的恶性肿瘤，兼具高发病率与高致死率。已有越来越多的研究证据表明，分选连接蛋白20（Sorting Nexin 20，SNX20）在人类癌症的发生进展中发挥关键调控作用。然而，SNX20在肺腺癌（Lung Adenocarcinoma，LUAD）中的具体功能与作用机制仍鲜为人知。本研究借助TCGA、GEO及CCLE数据库，检测了SNX20在多种人类癌症中的表达水平，结果显示SNX20在肺腺癌中呈低表达状态，且其表达水平与肺癌患者不良预后及肿瘤免疫细胞浸润程度呈显著正相关。本研究发现，过表达SNX20可显著抑制非小细胞肺癌（Non-Small Cell Lung Cancer，NSCLC）细胞的增殖与迁移能力。随后，本研究构建了LUAD中调控SNX20的相关分子网络，进一步机制研究证实，SNX20表达下调可能由DNA高甲基化所导致。此外，本研究证实SNX20AR/miRNA-301a-3p介导的SNX20表达下调，与肺腺癌中的肺癌进展及肿瘤免疫浸润密切相关。本研究结果提示，非编码RNA（non-coding RNAs，ncRNAs）在SNX20的调控网络中发挥关键作用。综上，本研究证实SNX20AR/miRNA-301a-3p/SNX20调控轴在肺腺癌中发挥抑癌作用，表明SNX20有望成为未来肺癌治疗的有效靶点。