Nanodynamo: studying the dynamics of RNA metabolism through mathematical modelling and native RNA profiling.

The combined action of coordinated nuclear and cytoplasmic steps shapes gene expression programs at steady state and determines their concerted response to perturbations. We developed Nanodynamo, an experimental and computational workflow for the comprehensive quantification of the kinetic rates of nuclear and cytoplasmatic steps of the RNA life cycle. Nanodynamo relies on mathematical modelling following the sequencing of native RNA from cellular fractions and polysomes.

协同核与细胞质的多步骤联合作用，不仅塑造了稳态条件下的基因表达程序，更决定了其对各类扰动的协同响应。我们开发了Nanodynamo，一套可全面定量RNA生命周期中核与细胞质各步骤动力学速率的实验与计算整合工作流程。该工具基于细胞组分与多聚核糖体来源的天然RNA测序结果开展数学建模。