B-cell lymphoma, NOS - Male, 50 - Cell image [3300730069482401]

This is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS cell sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format. The detailed information about the patient, sample, and diagnosis are as follows: Patient:Age: 50Clinical description: The material was taken from the region of the left anterior superior iliac spine. The patient is undergoing a diagnosis of the lymphoproliferative process. Immunophenotyping of blood and marrow suggests marginal zone lymphoma.Gender: MaleDiagnosis:Classification: ICD-O_3.2Classification code: MALIGNANT LYMPHOMAS NOS OR DIFFUSEDiagnosis: B-cell lymphoma, NOSResult of the histopathological examination: Trepanobioptate in two fragments of a total length of 23 mm. Cellularity is approximately 90%. The bone marrow shows infiltrates of medium to large B lymphocytes with phenotype: CD45(+), CD20(+), Bcl2(+), CD43(+/-), MUM1(+), c-myc(+/-, in about 30% of cells). Reactions with CD10, Bcl6, CD5, Cyclin D1, SOX11, Annexin A1, DBA44, Tdt, CD34, CD23 and MPO are negative. MIB1/Ki67 60%. The infiltrates described above represent about 80% of all cells. In residually preserved normal hematopoietic tissue, E: M ratio =1:2. Granulocytic lineage (MPO+) with rejuvenation. Blastic cells with CD34 expression represent approximately 1% of all cells. Red cell lineage (CD71+) depleted. Quite numerous megakaryocytes (CD61+). Focal reticulin fibers (MF-1). The microscopic picture supports the diagnosis of B-cell lymphoma of high malignancy. Together with the phenotype determined cytometrically and the clinical presentation (splenomegaly), this is most likely a progression/transformation of splenic lymphoma (SMZL).Sample:Material: FFPECollecting method: Bone marrow aspirationTopography: HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMSOrgan: HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMSTissue: Bone marrowType of staining: positive/HEStaining: Not applicableAntibody: Not applicableTechnology:Equipment: Pannoramic 250 3DHistechLens: 20xOrganization:Source: Medical University Gdańsk

本数据集为格但斯克医科大学（Medical University Gdańsk）获取、并保存于ZMDL-GUMED的造血与淋巴网状系统（HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS）细胞样本的组织病理学图像。样本图像采用Pannoramic 250 3DHistech玻片扫描仪拍摄（放大倍数20×），并以DICOM格式存储。

### 患者与诊断详情
患者年龄：50岁
临床描述：样本取自左髂前上棘区域。患者疑似存在淋巴增殖性病变，血液与骨髓免疫表型检测提示为边缘区淋巴瘤（Marginal Zone Lymphoma）。
患者性别：男性
诊断分类：ICD-O_3.2，分类编码：恶性淋巴瘤非特指型或弥漫性（MALIGNANT LYMPHOMAS NOS OR DIFFUSE）
确诊诊断：B细胞淋巴瘤非特指型
组织病理学检查结果：获取2段骨髓穿刺活检标本，总长度23mm。细胞丰富度约为90%。骨髓可见中到大的B淋巴细胞浸润，其免疫表型为：CD45(+)、CD20(+)、Bcl2(+)、CD43(+/-)、MUM1(+)、c-myc(+/-，约占细胞的30%)。CD10、Bcl6、CD5、Cyclin D1、SOX11、Annexin A1、DBA44、Tdt、CD34、CD23及MPO染色均呈阴性。MIB1/Ki67增殖指数为60%。上述浸润细胞约占所有细胞的80%。在残留的正常造血组织中，粒红比值（E:M）为1:2。粒细胞系（MPO+）增生活跃。表达CD34的原始细胞约占所有细胞的1%。红系细胞（CD71+）显著减少。可见较多巨核细胞（CD61+）。局灶性网状纤维增生（MF-1级）。镜下形态支持高恶性B细胞淋巴瘤的诊断。结合流式细胞术确定的免疫表型及临床表现（脾大），该病变极有可能为脾淋巴瘤（Splenic Marginal Zone Lymphoma, SMZL）的进展或转化。

### 样本详情
样本类型：福尔马林固定石蜡包埋（FFPE）样本
采集方式：骨髓穿刺
取材部位：造血与淋巴网状系统
所属器官：造血与淋巴网状系统
组织类型：骨髓
染色类型：阳性/HE染色
染色方案：无特殊要求
所用抗体：无
技术与设备信息：
扫描设备：Pannoramic 250 3DHistech
镜头放大倍数：20×
提供机构：格但斯克医科大学