Single-oocyte mRNA sequencing reveals that AMH-overexpression alters oocyte gene expression. Single-oocyte mRNA sequencing reveals that AMH-overexpression alters oocyte gene expression

Research Question: Does over-expression of AMH lead to changes in oocyte gene expression in mice? Design: Naturally ovulated oocytes were dissected from the oviduct of wild-type, and AMH-overexpressing (Thy1.2-AMHTg/0) mice and single oocytes underwent transcriptome sequencing (n=11 and n=10, respectively). Results: Oocytes from Thy1.2-AMHTg/0 females displayed 39 significantly expressed differentially expressed genes (DEGs) compared to wild-type mouse oocytes. The most prominent gene expression changes were a 70-fold increase in Rpsa3a, a 22-fold increase in Hormad1 and a 5.6-fold increases in Fnip2 in the oocytes from Thy1.2-AMHTg/0 mice. Gene ontology analysis indicated that among the DEGs, the most enriched pathways were mitochondrial genes and DNA repair genes. Conclusions: These data show that overexpression of AMH, through its actions on granulosa cells, can lead to changes in the expression of specific genes in mouse oocytes. Overall design: RNA-seq profiling of individual, naturally ovulated oocytes obtained from either wild-type or Thy1.2-AMHTg/0 (AMH-overexpressing) female mice.

研究问题：抗缪勒管激素（AMH，Anti-Müllerian Hormone）过表达是否会导致小鼠卵母细胞基因表达发生改变？
实验设计：从野生型与AMH过表达（Thy1.2-AMHTg/0）小鼠的输卵管中分离自然排卵的卵母细胞，对单个卵母细胞开展转录组测序，野生型组与AMH过表达组的样本量分别为n=11与n=10。
研究结果：与野生型小鼠卵母细胞相比，Thy1.2-AMHTg/0雌性小鼠的卵母细胞中共存在39个显著差异表达基因（DEGs，Differentially Expressed Genes）。其中最显著的表达变化为：Thy1.2-AMHTg/0小鼠卵母细胞中Rpsa3a表达上调70倍、Hormad1表达上调22倍，Fnip2表达上调5.6倍。基因本体（GO，Gene Ontology）分析显示，差异表达基因富集程度最高的通路为线粒体相关基因与DNA修复相关基因。
研究结论：上述数据表明，AMH过表达可通过作用于颗粒细胞，引发小鼠卵母细胞特定基因的表达改变。
整体实验设计：对来自野生型或Thy1.2-AMHTg/0（AMH过表达）雌性小鼠的单个自然排卵卵母细胞进行RNA测序（RNA-seq）转录组分析。