CD95L derived si- and shRNAs kill cancer cells through an RNAi mechanism by targeting survival genes [shL1.RNAseq.lg]. Homo sapiens

We provide evidence that shRNAs and siRNAs derived from CD95 and CD95L preferentially target the 3' UTRs of survival genes culminating in a very robust mode of cell death we call DISE (Death Induced by Survival gene Elimination) Overall design: 293T cells were infected with either pTIP-shScr or pTIP-shL1 and following puromycin selection RNA was analyzed by deep sequencing 100hrs after addition of doxycycline

本研究提供实验证据表明，源自CD95与CD95L的短发夹RNA（shRNAs）和小干扰RNA（siRNAs）能够优先靶向生存基因的3'非翻译区（3' UTRs），进而诱导出一种极强效的细胞死亡模式，我们将其命名为DISE（Death Induced by Survival gene Elimination，即生存基因消除诱导死亡）。实验整体设计：将293T细胞分别感染pTIP-shScr或pTIP-shL1载体，经嘌呤霉素筛选后，于添加多西环素100小时时提取RNA并通过深度测序进行分析。