The effects of increased temperature on the physiological variable (heart rate, respiratory frequency, oxygen consumption, blood pressure, etc) of 2 Antarctic fish: Pagothenia borchgrevinki, Trematomus bernacchii

The effects of increased temperature on physiological variables in two Antarctic fish Pagothenia borchgrevinki (pelagic) and Trematomus bernacchii (sedentary) was investigated using a new technique to measure heart rate (ECG). Measurement of heart rate, respiratory frequency, and from these the respiratory/cardiac couplings in animals at rest and under stress were made. Animals were placed in a closed box respirometer and oxygen consumption was measured. The data also allowed analysis of heart rate variability and determining control of the heart rate by sympathetic/parasymphathetic nervous input. Using cannulated fish, heart rate and blood pressure was recorded in fish acutely raised to 3, 5 and 10°C. Blood samples were analysed for haematocrit, haemoglobin and hormone (adrenaline, noradrenalin) concentrations.

本研究采用新型心电图（ECG）心率测量技术，探究了升温对两种南极鱼类Pagothenia borchgrevinki（远洋型）与Trematomus bernacchii（定居底栖型）生理指标的影响。实验测定了个体在静息及应激状态下的心率、呼吸频率，并据此分析了呼吸-心脏耦合特征。将实验对象置于封闭式箱式呼吸代谢仪中，测定其耗氧量。所得数据集还可用于分析心率变异性，以及探究交感与副交感神经输入对心率的调控机制。本研究通过经插管处理的鱼类，对急性升温至3℃、5℃和10℃的个体的心率与血压进行了记录。采集血液样本以分析血细胞比容、血红蛋白浓度及肾上腺素、去甲肾上腺素等激素的浓度。