Sequencing result of a mixture of synthesized DNA barcodes

Raw data for the Nature Protocol manuscript entitled "clonal tracking using embedded viral barcoding and high-throughput sequencing".Embedded viral barcoding in combination with high throughput sequencing is a powerful technology to track single cell clones. It can provide clonal level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy. Here, we present a detailed protocol of a viral barcoding procedure including the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing data. The entire procedure can becompleted within a few weeks. This barcoding method requires cells to be susceptible to viral transduction. It provides high sensitivity and throughput, and allows precise quantification of cellular progeny. It is cost efficient and does not require any advanced skills. It can also be easily adapted to many types of applications, including both in vitro and in vivo experiments.

本数据集为一篇题为《基于嵌入式病毒条形码（viral barcoding）与高通量测序（high-throughput sequencing）的克隆追踪（clonal tracking）》的《自然-实验方法》（Nature Protocols）研究手稿的配套原始数据。嵌入式病毒条形码技术结合高通量测序是一种用于追踪单细胞克隆的强有力技术，可在克隆层面解析细胞增殖、发育、分化、迁移及治疗疗效。本文提供一套详尽的病毒条形码操作流程方案，涵盖条形码文库构建、条形码病毒递送、条形码回收以及条形码测序数据的计算分析。整套流程可在数周内完成。该条形码技术要求细胞对病毒转导（viral transduction）具有易感性，具备高灵敏度与高通量特性，可实现对细胞子代的精准定量。该方法成本效益优异，且无需任何高级专业技能，同时可轻松适配多种应用场景，涵盖体外（in vitro）与体内（in vivo）实验。