Clca1 expression in the aged kidney in mice

In this study, a discovery approach was designed to identify novel mRNA transcripts that may be involved in kidney aging. RNA-Seq revealed increase in the expression of Clca1 mRNA in the aged kidney. CLCA1 facilitates Cl- current operated by TMEM16A, a Ca++-dependent Cl- channel. Cl- movement accompanied by cations such as Na+ and K+ or in exchange for other anions occurs among fluid compartments in many segments of the nephron. Cl- shift among these compartments is governed by electrochemical potential and transporters and exchangers.

本研究设计了一种发现式筛选方法，用于鉴定可能参与肾脏衰老过程的新型信使核糖核酸（mRNA）转录本。RNA测序（RNA-Seq）结果显示，衰老肾脏中Clca1 mRNA的表达水平显著升高。CLCA1可促进由TMEM16A——一种Ca++依赖性Cl-通道——所介导的Cl-电流。在肾单位的多个节段中，Cl-可伴随Na+、K+等阳离子跨体液腔室移动，或与其他阴离子进行交换转运。此类腔室间的Cl-位移由电化学电位以及各类转运蛋白与交换蛋白共同调控。