cold stress -Impact of cold stress on grapevine inflorescence. Vitis vinifera

12plex_vitis_2012_04 - cold stress - In temperate species, low temperatures adversely affect plant growth and development, constraining spatial expansion and productivity. Cold can trigger adaptive process or lead to alterations in physiological traits when stress intensity exceeds a certain threshold. Grapevine flower development and fruit set are influenced by cold nights in the vineyard. The correct formation of sexual organs and the success of reproduction are dependent upon sugar supply which can be affected by low temperatures. Many pathways of carbon metabolism may be affected by low temperatures however limited information is available about fluctuations in carbon metabolism in inflorescences. - Experiments were performed on inflorescence of Vitis vinifera L. cv. Pinot noir fruiting cuttings. First, inflorescences at female meiosis were collected the day of the experiment, then cuttings with the inflorescence were placed at 0°C for a 8 h night. Control plants were maintained in a growth chamber for 8 h at 19°C. Inflorescences were collected after 1 h and 8 h of cold. Additional time point was performed 2 h after the end of the cold night by placing the inflorescence at 25°C after the end of the cold night. Control plants were also collected. Overall design: 12 dye-swap - treated vs untreated comparison

12plex_vitis_2012_04——低温胁迫
对于温带植物而言，低温会对其生长发育产生显著不利影响，限制植株的空间拓展与生物生产力。当胁迫强度突破特定阈值时，低温既可诱导植物产生适应响应，也可引发其生理性状发生改变。葡萄园中的夜间低温会影响葡萄的花器官发育与坐果。有性生殖器官的正常形成与繁殖成功，依赖于糖类物质的供给，而低温会对糖类供给产生负面影响。诸多碳代谢通路均可能受低温影响，但目前针对花序碳代谢波动的相关研究仍较为有限。
本实验以酿酒葡萄（Vitis vinifera L.）品种黑皮诺（Pinot noir）的扦插结果枝花序为实验材料。实验当日，先采集处于雌性减数分裂阶段的花序；随后将带花序的扦插枝置于0℃环境中，进行8小时的夜间低温胁迫处理。对照组植株则置于19℃的人工气候箱中培养8小时。分别在低温处理1小时、8小时后采集花序样本。额外设置1个恢复培养采样点：在夜间低温处理结束后，将花序转移至25℃环境下培养2小时后再行采样。对照组植株亦同步完成采样。
实验整体设计：采用12次染料互换（dye-swap）实验方案，开展处理组与对照组的配对比较。