Leukemia associated mutant Wilms' tumour gene 1 protein promotes expansion of human hematopoietic progenitor cells

Analysis of gene expression levels in hematopoietic progenitor cells retrovirally transduced with full-length WT1(+/-) or a WT1-mutant lacking zinc-finger, WT1(delZ) cultured in vitro for 14 days. The hypothesis tested in this study was that the WT1-mutant confers an increased proliferation rate after 14 days in culture and an erythroid phenotype. Results provide information of upregulation of some genes associated with cellcycle progression, upregulated genes connected with erythropoiesis and downmodulation of some genes associated with myeloid differentiation. Total RNA obtained from CD34+ progenitor cells in suspension cultures for 14 days.

本研究对经逆转录病毒转导全长WT1(+/-)或缺失锌指结构(zinc-finger)的WT1突变体WT1(delZ)、并经体外培养14天的造血祖细胞(hematopoietic progenitor cells)的基因表达水平进行了分析。本研究验证的科学假说为：该WT1突变体可使体外培养14天后的细胞增殖速率提升，并诱导红系表型。研究结果揭示了若干与细胞周期进程相关基因的上调表达、与红细胞生成(erythropoiesis)相关的上调基因，以及若干与髓系分化(myeloid differentiation)相关基因的表达下调。实验所用总RNA提取自经14天悬浮培养的CD34+祖细胞。