Galpha13 restricts nutrient driven proliferation in mucosal germinal centers

RNA sequencing of control or GNA13-deficient NUDUL1 cells treated with U46619 to stimulate Galpha13 signaling for 24 hours. Single cell RNAsequencing of sorted WT or Gna13-deficient germinal center B cells from peripheral or mesenteric lymph nodes. Heavy chain BCR repertoire of sorted WT or Gna13-deficient germinal center B cells from mesenteric lymph nodes. Whole genome CRISPR/Cas9 screen of NUDUL1 or OCI-Ly8 cells treated +/- U46619 to stimulate Galpha13 signaling.经U46619刺激Gα13信号通路（Galpha13 signaling）处理24小时的对照组与GNA13缺陷型NUDUL1细胞的RNA测序（RNA sequencing）数据集。 从外周及肠系膜淋巴结中分选获得的野生型（Wild Type, WT）或GNA13缺陷型生发中心B细胞的单细胞RNA测序（single cell RNA sequencing）数据集。 从肠系膜淋巴结中分选获得的野生型（WT）或GNA13缺陷型生发中心B细胞的B细胞受体（BCR, B cell receptor）重链库数据集。 经U46619刺激Gα13信号通路处理或未处理的NUDUL1与OCI-Ly8细胞的全基因组CRISPR/Cas9筛选（CRISPR/Cas9 screen）数据集。