Data_Sheet_3_Surveillance and genetic characterization of Listeria monocytogenes in the food chain in Montenegro during the period 2014–2022.PDF

IntroductionListeria monocytogenes is an ubiquitous foodborne pathogen that represents a serious threat to public health and the food industry.
MethodsIn this study Whole Genome Sequencing (WGS) was used to characterize 160 L. monocytogenes isolates obtained from 22,593 different food sources in Montenegro during the years 2014–2022.
ResultsIsolates belonged to 21 different clonal complexes (CCs), 22 sequence types (STs) and 73 core genome multilocus sequence types (cgMLST) revealing a high diversity. The most prevalent STs were ST8 (n = 29), ST9 (n = 31), ST121 (n = 19) and ST155 (n = 20). All isolates carried virulence genes (VGs), 111 isolates carried mobile genetic elements (MGEs) (ranging from 1 to 7 MGEs) and 101 isolates carried plasmids (ranging from 1 to 3 plasmids). All isolates carried the intrinsic resistance genes fosX and lin. None of the isolates carried acquired antimicrobial resistance genes (ARGs).
Discussion/conclusionContinuous monitoring and surveillance of L. monocytogenes is needed for improving and ameliorating the public health.

引言：单核细胞增生李斯特菌（Listeria monocytogenes）是一种广泛分布的食源性致病菌，对公共卫生与食品工业均构成严重威胁。
材料与方法：本研究采用全基因组测序（WGS）技术，对2014年至2022年间从黑山22593份不同食品来源中分离得到的160株单核细胞增生李斯特菌进行分型鉴定。
结果：本次研究的分离株分属于21种不同的克隆复合体（CCs）、22种序列型（STs）以及73种核心基因组多位点序列分型（cgMLST），展现出较高的遗传多样性。其中优势序列型为ST8（n=29）、ST9（n=31）、ST121（n=19）与ST155（n=20）。所有分离株均携带毒力基因（VGs）；111株携带移动遗传元件（MGEs），携带数量介于1至7个不等；101株携带质粒，数量介于1至3个不等。所有分离株均携带固有耐药基因fosX与lin，未检出任何获得性抗菌药物耐药基因（ARGs）。
讨论与结论：为优化与完善公共卫生体系，需持续开展单核细胞增生李斯特菌的监测与防控工作。