Transcriptional expression level during exponential growth phase in Kluyveromyces marxianus. Kluyveromyces marxianus

To understand functional roles of ncRNAs during exponential growth phase, we captured transcriptome changes by conducting strand-specific RNA-seq. Overall design: Directional RNA-seq (dUTP method by TruSeq®) were generated in two biological replicates by using Illumina MiSeq

为探究指数生长期中非编码RNA（non-coding RNAs, ncRNAs）的功能角色，本研究通过开展链特异性RNA测序（strand-specific RNA-seq）捕获转录组的动态变化。整体实验设计：采用基于TruSeq®试剂盒的dUTP法构建定向RNA测序文库，设置2次生物学重复，测序平台为Illumina MiSeq。