Sequencing and analysis of three plant growth promoting rhizobacteria isolated from the rhizosphere of plantation crops coconut, cocoa and arecanut. Sequencing and analysis of three plant growth promoting rhizobacteria

Coconut, cocoa and arecanut are commercial plantation crops that play a vital role in the Indian economy. We sequenced three plant growth promoting rhizobacteria from coconut (CPCRI-1), cocoa (CPCRI-2) and arecanut (CPCRI-3) respectively. The draft genome sequences for CPCRI-1 [4.7 Mb], CPCRI-2 [5.9 Mb] and for CPCRI-3 [5.1 Mb] encode 4056, 4637 and 4286 protein-coding genes, respectively. CPCRI-1 and CPCRI-3 belong to Enterobacteriaceae family and CPCRI-2 groups with Pseudomonadacea family. Genomes of all three bacteria contain genes for central carbohydrate metabolism, indole acetic acid production and quorum sensing indicating the presence of plant growth promoting traits. Functional annotation comparison identified several differentially enriched groups. For example, CPCRI-2 genome contains higher percentage of transcription initiation and regulation and siderophore pyoverdin genes compared to the CPCRI-1 and CPCRI-3. CPCRI-2 genome contains several genes involved in the degradation of various aromatic compounds such as benzoate, tetrachloroethane, bisphenolA etc. CPCRI-1 genome contains a large number of genes associated with flagella and pili biosynthesis as compared to CPCRI-2 and CPCRI-3 genomes. Genome sequences of these bacteria will enhance our understanding of the interactions between these rhizobacteria and their respective crops. This will help in designing sustainable agriculture strategies by using rhizobacteria as growth promoting agents.

椰子、可可与槟榔均为商业化种植作物，在印度经济中发挥着关键作用。本研究分别对来源于椰子（CPCRI-1）、可可（CPCRI-2）及槟榔（CPCRI-3）的3株植物促生根际细菌（plant growth promoting rhizobacteria）进行了测序。三者的草图基因组序列长度分别为4.7 Mb、5.9 Mb与5.1 Mb，各自编码4056、4637和4286个蛋白质编码基因。其中，CPCRI-1与CPCRI-3隶属于肠杆菌科（Enterobacteriaceae），而CPCRI-2归属于假单胞菌科（Pseudomonadaceae）。三株细菌的基因组均携带参与中央碳水化合物代谢、吲哚乙酸合成以及群体感应的基因，这表明它们具备植物促生相关特性。通过功能注释对比分析，研究发现了多个差异富集的功能类群：例如，相较于CPCRI-1与CPCRI-3，CPCRI-2基因组中转录起始与调控相关基因以及嗜铁素pyoverdin编码基因的占比显著更高；CPCRI-2基因组还包含多组参与降解各类芳香族化合物的基因，如苯甲酸、四氯乙烷、双酚A等；而相较于CPCRI-2与CPCRI-3，CPCRI-1基因组中存在大量与鞭毛及菌毛生物合成相关的基因。上述细菌的基因组序列将加深我们对这些根际细菌与其宿主作物之间互作关系的理解，进而助力以根际细菌为促生因子构建可持续农业策略。