Copy number analysis of spontaneously transformed hTERT-immortalized ovarian surface epithelial cells. Homo sapiens

We present evidence for cytogenetic changes in two transformed ovarian surface epithelium cell lines, TOSE1 and TOSE4. hTERT-immortalised ovarian surface epithelial cell line spontaneously transformed as measured by growth in soft agar. These cells were grown on plastic, and genomic DNA was extracted and analyzed for cytogenetic changes. Overall design: The genotyping Affymetrix SNP6.0 arrays were used to define copy number changes in the TOSE1 and TOSE4 cell lines.

本研究报道了两株转化型卵巢表面上皮细胞系TOSE1与TOSE4的细胞遗传学改变相关证据。两株经人类端粒酶逆转录酶（hTERT）永生化的卵巢表面上皮细胞系可通过软琼脂集落形成实验证实发生自发转化。将上述细胞接种于塑料培养表面进行培养，随后提取基因组DNA并开展细胞遗传学改变相关分析。实验整体设计：本研究采用Affymetrix SNP6.0基因分型芯片对TOSE1与TOSE4细胞系的拷贝数变异进行鉴定。