A transposon insertion drove the loss of natural seed shattering during foxtail millet domestication

To identify how the sh1 gene controls the downstream genes for shattering, RNA-seq was then conducted from the yong panicles two days after heading of the transgenic edited plant (Csh1-1) with an 1-bp-insertion editing mutation of a gene-frame shift and the non transgenic control plant CK (ME034v)

为阐明sh1基因（sh1 gene）调控落粒相关下游基因的分子机制，本研究对携带1bp插入引发移码编辑突变的转基因编辑植株Csh1-1以及非转基因对照植株CK（ME034v）在抽穗后两天的幼穗样本开展了RNA测序（RNA-seq）