Mitochondria-targeted partial inhibiton of complex I increase health and lifespan in mice.

We performed next-generation RNA sequencing (RNA-seq) using brain tissue from 23 months old vehicle and CP2-treated non-transgenic (NTG) and Young (3 months old) WT mice. By comparing transcriptomic data of vehicle and CP2-treated NTG_Old mice, we found processes affected by CP2 such as genes involved in regulation of circadian rhythm, immune system, oxidant detoxifications. In comparison to Young mice, CP2 treatment in NTG_Old mice downregulated the expression of gene sets involved in regulation of oxidative stress and lipid metabolism, which were both upregulated with old age in vehicle-treated NTG_Old mice.

我们采用23月龄溶剂对照组及CP2处理组的非转基因（non-transgenic, NTG）老龄小鼠、3月龄野生型（wild type, WT）年轻小鼠的脑组织，开展下一代RNA测序（RNA-seq）。通过对比溶剂对照组与CP2处理组老龄非转基因小鼠的转录组数据，我们发现CP2可影响诸多生物学过程，包括参与昼夜节律调控、免疫系统调节及氧化解毒的相关基因。与年轻小鼠相比，CP2处理的老龄非转基因小鼠中，参与氧化应激调控与脂质代谢的基因集表达出现下调；而在溶剂处理的老龄非转基因小鼠体内，这两类基因集的表达均随衰老进程而上调。