Impacts of Polystyrene Microplastics on Skeletal Development in Zebrafish Larvae

Total RNA was extracted from 1 g of bone tissue samples collected from the control group and the 20 mg/L polystyrene microplastics exposure group on day 28. The concentration and purity of the extracted RNA samples were determined using a NanoDrop 2000, and their integrity was assessed by agarose gel electrophoresis. The RNA quality was measured using an Agilent 5300 platform (RQN). Gene differential expression analysis was performed between the zebrafish control group and the 20 mg/L polystyrene microplastics exposure group to identify differentially expressed genes, which were annotated using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the Gene Ontology (GO) database .

于第28天，分别从对照组与20 mg/L聚苯乙烯微塑料暴露组采集的1 g骨组织样本中提取总核糖核酸（RNA）。采用NanoDrop 2000分光光度计测定所提取RNA样本的浓度与纯度，并通过琼脂糖凝胶电泳评估其完整性；借助安捷伦5300平台（RQN）检测RNA质量。以斑马鱼对照组与20 mg/L聚苯乙烯微塑料暴露组为研究对象开展基因差异表达分析，筛选得到差异表达基因；随后采用京都基因与基因组百科全书（KEGG）数据库及基因本体（GO）数据库对上述差异表达基因进行功能注释。