ADDOMEx Tier 3 Experiments: Mesocosm #3 Gulf of Mexico open ocean waters (GOMOO) and Mesocosm #4 Gulf of Mexico coastal waters (GOMCOAST) 0.2 um filter size

To determine the effects of oil and dispersant on eukaryotic microbial communities, mesocosm experiments were run with natural microbial communities from both an open ocean and coastal site. Samples were taken every 12 hours and filtered onto 0.2 um pore size filters to determine the large size fraction of the microbial community. Samples were then extracted for DNA, amplified using Bradley et al., 2016 primers for the 18S rRNA V8/V9 region and submitted for sequencing. Data include the raw paired end reads from this analysis and was later processed using the bioinformatics tool, mothur. Data were combined with the 10um size fraction for whole community analysis throughout the experiment.

为探究石油与分散剂对真核微生物群落（eukaryotic microbial communities）的影响，研究人员采用来自开阔海洋及沿海站点的自然微生物群落开展中宇宙实验（mesocosm experiments）。每12小时采集一次样本，经0.2微米孔径滤膜过滤以获取微生物群落的大尺寸组分。随后对样本进行DNA提取，使用Bradley等人2016年报道的引物扩增18S rRNA V8/V9区域，并提交测序。数据集包含该分析产生的原始双端测序reads（paired end reads），后续通过生物信息学工具mothur进行处理。为开展实验全程的全群落分析，本数据集还整合了10微米尺寸组分的数据。