Differential gene expression profiling of urinary bladder wall under resiniferatoxin influence. Genes identification involved in RTX influence in the urinary bladder

Resiniferatoxin (RTX) is a potent capsaicin analogue used as a drug for experimental therapy of a range of neurogenic disorders associated with enhanced nociceptive transmission, including lower urinary tract symptoms. The study investigated the transcriptomic profile of the control and RTX-treated porcine urinary bladder wall. The analysis was performed on 12 juvenile female pigs. Six pigs served as controls and were treated with intravesical instillation of a 5% aqueous solution of ethyl alcohol. Another group of 6 pigs was treated with RTX by intravesical instillation of the toxin. One week after the administration of the aqueous solution of ethyl alcohol or RTX, a midline laparotomy was performed in all the animals, and samples of the urinary bladder wall were cut out for transcriptome analysis. Within both the experimental and control groups total RNA was isolated and high-throughput RNA sequencing was used to analyze the global changes in the transcriptome of the collected parts of the urinary bladder after RTX treatment.

树脂毒素（Resiniferatoxin，RTX）是一种强效辣椒素类似物，被用作治疗多种与增强型伤害性感受传递相关的神经源性疾病的实验性药物，其中包括下尿路症状。本研究对对照组及经RTX处理的猪膀胱壁的转录组谱进行了分析。实验共使用12只幼年雌性猪：其中6只作为对照组，接受5%乙醇水溶液的膀胱内灌注处理；另外6只则通过膀胱内灌注该毒素的方式接受RTX处理。在给予乙醇水溶液或RTX一周后，对所有实验动物实施正中剖腹术，截取膀胱壁样本以用于转录组分析。两组实验对象均提取总RNA，并采用高通量RNA测序技术，分析RTX处理后采集的膀胱壁样本转录组的全局表达变化。