Derriere targets in Xenopus posterior mesoderm. Xenopus laevis

Left-right axis determination is a fundamental and conserved developmental process, with consequences for human pathology. At the molecular level, this process requires at least two centers of nodal-type TGFbeta signals: the first center, containing nodal/Xn Microarrays were used to determine differences in gene expression in posterior mesoderm of Xenopus embryos. We compared controls and Derriere depleted embryos. Keywords: loss of function Overall design: Microarrays were used to determine differences in gene expression in posterior mesoderm of Xenopus embryos. We compared controls and embryos depleted of Derriere protein using MO oligonucleotides.

左右轴确定是一项基础且保守的发育过程，其异常与人类病理状态密切相关。在分子层面，该过程至少需要两类结蛋白（Nodal）型转化生长因子β（TGFβ）信号中心，首个中心包含结蛋白（Nodal）/爪蟾结蛋白（Xn）。本研究使用微阵列（Microarrays）技术检测非洲爪蟾胚胎后部中胚层的基因表达差异，比较对照组与Derriere基因敲低胚胎。关键词：功能缺失。实验设计：使用微阵列（Microarrays）技术检测非洲爪蟾胚胎后部中胚层的基因表达差异，比较对照组与使用吗啉代寡核苷酸（MO oligonucleotides）敲低Derriere蛋白的胚胎。