Gene expression profile in breast cancer cells

We report the gene expression patterns in MDA-MB-231 (a line selected for low metastatic ability), MDA-MB-231-1833 (its bone-tropic metastatic derivative line), MDA-MB-231p27CK-DD (a phosphomimetic cell line), MDA-MB-231-1833shp27 (p27 knockdown cell line), MDA-MB-231-1833PF1502 (PI3K inhibitor treatment). It shows that the gene expression pattern are regulated in a p27 phosphorylation-dependent manner. Overall design: Examination in 5 cell types.

本研究报道了下述细胞系的基因表达谱：MDA-MB-231（一株经筛选获得的低转移能力细胞系）、MDA-MB-231-1833（其亲代细胞的骨转移性衍生细胞系）、MDA-MB-231p27CK-DD（磷酸化模拟细胞系）、MDA-MB-231-1833shp27（p27基因敲低细胞系）以及MDA-MB-231-1833PF1502（经磷脂酰肌醇3-激酶（PI3K）抑制剂处理的细胞系）。研究结果表明，基因表达谱以p27磷酸化依赖的方式受到调控。整体实验设计：对5种细胞类型进行检测分析。