P. syringae pv. phaseolicola NPS3121 responses to bean leaf extract

Many genes involve in pathogenicity and virulence are induced only in plant or in the presence of host components. Bean leaf extract was obtained from healthy bean leaves. In this work we investigated the effect of bean leaf extract on the transcriptomic profile of the bacterium, when grown at low temperature in minimal medium with or without extract from healthy bean leaves. Two RNA samples were compared, one prepared from cells grown in minimal medium and the other from cells grown in minimal medium supplemented with bean leaf extract. To control de biological variation that might interfere with data interpretation, a minimum of three biological replicates were prepared and two technical replicates with the same RNA (swap).

许多参与致病性与毒力的基因仅在植物宿主内或宿主组分存在时才会被诱导表达。本研究的菜豆叶片提取物取自健康菜豆叶片。
本研究探究了低温培养条件下，菜豆叶片提取物对该细菌转录组谱的影响，实验所用培养基分为两组，分别添加健康菜豆叶片提取物与不添加提取物。本研究对两组RNA样本进行了比对：一组取自基础培养基培养的细菌细胞，另一组取自添加菜豆叶片提取物的基础培养基培养的细菌细胞。为控制可能干扰数据解读的生物学变异，本研究至少设置3次生物学重复，并针对同一RNA样本开展2次技术重复（样本互换实验）。