AGO-bound virus-derived RNA in Influenza infected WT and AGO4 knockout bone marrow derived macrophages performed by Immunoprecipitation following by High Throughput Sequencing. AGO-bound virus-derived RNA in Influenza infected WT and AGO4 knockout bone marrow derived macrophages performed by Immunoprecipitation following by High Throughput Sequencing

We identified virus-derived RNA bound to AGO in Influenza A or Influenza delNS1-infected macrophages taken from wild-type or Ago4 KO littermate control mice Overall design: Immunoprecipitation of AGO-bound RNA after UV crosslinking using pan AGO antibody in WT or Ago4 KO primary bone marrow-derived macrophages infected with Influenza A (PR/8/1934) or A/PR/8/1934 ΔNS1 (MOI 1 for 24h)

我们在取自野生型（WT）或Ago4敲除（Ago4 KO）同窝对照小鼠的、经甲型流感病毒（Influenza A）或甲型流感delNS1病毒感染的巨噬细胞中，鉴定出了与AGO（Argonaute蛋白）结合的病毒来源RNA。实验设计：经紫外交联处理后，使用泛AGO抗体对野生型（WT）或Ago4敲除原代骨髓来源巨噬细胞中的AGO结合RNA进行免疫沉淀；上述巨噬细胞分别感染了甲型流感病毒PR/8/1934株或A/PR/8/1934 ΔNS1株，感染复数（MOI）为1，感染时长为24小时。