Deciphering Gene Expression Program of MAP3K1 in Mouse Eyelid Morphogenesis

Embryonic eyelid closure is a critical step of mammalian ocular surface development involving the forward movement and ultimate fusion of the upper and lower eyelids. Although its underlying mechanism of action is not fully understood, a functional mitogen-activated protein kinase kinase kinase 1 (MAP3K1) is essentially required for eyelid closure. We used microarray to characterize the central role that MAP3K1 plays in embryonic eyelid closure. We used Laser Capture Microdissection (LCM) to obtain highly enriched eyelid leading edge and inner eyelid cells from E15.5 wild type and MAP3K1-deficient fetuses and analyze genome-wide expression profiles.

胚胎眼睑闭合是哺乳动物眼表发育的关键步骤，该过程涉及上下眼睑向前迁移并最终融合。尽管其潜在作用机制尚未完全阐明，但功能性丝裂原活化蛋白激酶激酶激酶1（MAP3K1）是眼睑闭合所必需的核心因子。本研究通过基因芯片技术解析了MAP3K1在胚胎眼睑闭合过程中的核心作用。我们采用激光捕获显微切割（LCM）技术，从E15.5野生型及MAP3K1缺陷胎鼠中分离得到高度富集的眼睑前缘细胞与眼睑内层细胞，并开展全基因组表达谱分析。