Phenazines regulated genes in Pseudomonas chlororaphis 30-84

Pseudomonas chlororaphis strain 30-84 is an effective biological control agent against take-all disease of wheat. In this study, we conducted an RNA-seq analysis by comparing the wild type strain, PCA and O star with a phenazine deficient mutant. RNA-seq analysis identified over 800 genes differentially regulated by phenazines. Overall design: A total of 8 samples were analyzed in AB medium + 2% casamino acids, Pseudomonas chlororaphis wild type strain (2 replicates); Pseudomonas chlororaphis ZN mutant (2 replicates); Pseudomonas chlororaphis PCA strain (2 replicates); Pseudomonas chlororaphis O star (2 replicates).

绿针假单胞菌30-84菌株（Pseudomonas chlororaphis strain 30-84）是一种可有效防治小麦全蚀病（take-all disease of wheat）的生物防治剂（biological control agent）。本研究通过对比野生型菌株、PCA菌株、O star菌株与吩嗪缺陷突变株，开展了RNA测序（RNA-seq）分析。RNA-seq分析共鉴定出800余个受吩嗪（phenazine）类物质调控的差异表达基因。实验设计概述：本研究在AB培养基（AB medium）添加2%酪蛋白氨基酸（casamino acids）的条件下培养供试菌株，共设置8个样本进行检测，具体分组如下：绿针假单胞菌野生型菌株（2次生物学重复）、绿针假单胞菌ZN突变株（2次生物学重复）、绿针假单胞菌PCA菌株（2次生物学重复）以及绿针假单胞菌O star菌株（2次生物学重复）。