One-day old broilers exposed to amoxicillin

The objective of this study was to investigate the impact of short-term antibiotic treatment in early life on both early microbial colonization of the gut and functional development of intestinal tissue. From both control and antibiotic treated birds intestinal content samples were taken for microbiota analyzes and intestinal tissue samples were extracted for gene expression analyzes, both at three subsequent time-points (days 1, 5, and 14). 1-day-old chicks (Cobb500) were housed in a floor pen system in which the chicks had ad libitum access to feed and water. At day 1, 2 groups were created; control and antibiotic treated (amoxicillin, 24h in drinking water). At days 1, 80 birds of the control group were sacrificed for tissue sampling. At days 5 and 14, per time-point 80 birds of the control group and 80 birds of the antibiotic treated group were sacrificed for tissue sampling. Subsequently, samples from these 80 birds were pooled in 8 pools of ten birds.

本研究旨在探究生命早期短期抗生素干预对肠道早期微生物定植以及肠道组织功能发育的影响。研究分别从对照组与抗生素处理组禽类中采集肠道内容物样本用于微生物群分析，并提取肠道组织样本开展基因表达分析，所有采样均在三个连续时间点（第1、5、14日）完成。试验所用的1日龄科宝500（Cobb500）肉鸡雏鸡饲养于平养栏系统中，雏鸡可自由采食与饮水。试验第1日将雏鸡分为两组：对照组与抗生素处理组（阿莫西林以24小时持续给药的方式添加于饮水中）。第1日时处死对照组80只雏鸡用于组织采样；在第5日与第14日两个时间点，每个时间点分别处死对照组与抗生素处理组各80只雏鸡用于组织采样。随后将每个时间点采集的80只雏鸡的样本混合为8个混合样本，每个混合样本包含10只雏鸡的组织样本。