FOXC2 knockdown in MDA-MB-231 cells

To determine gene expression changes in vasculogenic mimicry competent human breast cancer cells with loss of FOXC2 we performed RNA-seq of MDA-MB-231 cells with FOXC2 knockdown. MDA-MB-231 cells were transduced with a control shRNA (shREN) or two different FOXC2-targetting shRNAs (shFOXC2#1 and shFOXC2#3). Two biological replicates per shRNA were subjected to RNA-seq analysis.为探究具备血管生成拟态（vasculogenic mimicry）能力的人乳腺癌细胞在FOXC2缺失后的基因表达变化，我们对FOXC2敲低的MDA-MB-231细胞开展了RNA测序（RNA-seq）分析。实验中，将MDA-MB-231细胞分别转导以对照短发夹RNA（shRNA）shREN，以及两种靶向FOXC2的短发夹RNA（shFOXC2#1和shFOXC2#3）；针对每种shRNA，我们设置了两个生物学重复，并对其进行RNA测序分析。