Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool117_h1930004_TH-TL. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool117_h1930004_TH-TL

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人脑的细胞架构，对于解析其多样功能与复杂的动物行为至关重要。本研究对人脑多个区域开展单细胞核甲基化测序（single nucleus methylation sequencing），成功识别出多种不同的细胞簇，并构建了层级分类体系。该体系涵盖的细胞簇既包含已知的主要脑细胞类型，也存在潜在亚型。我们依托这批实验数据，对每种细胞簇的表观基因组特征进行了鉴定，并明确了其特异性调控元件。整体实验设计：对脑区实施单细胞核甲基化测序3（snmC-seq3），以解析成人脑的表观基因组图谱。