Neurofibromin (Nf1) controls metabolic balance and Notch-dependent quiescence of murine juvenile myogenic progenitors [RNA-seq]

Purpose: To study Nf1 regulated pathways in controlling juvenile satellite cells quiescence induction. Methods: We sequenced total mRNAs isolated from juvenile satellite cells FACS sorted from postnatal day 7 Control (Nf1flox/+ Myf5Cre+) and KO (Nf1flox/flox Myf5Cre+) mouse hindlimb skeletal muscle. Results: Our study suggests that Nf1 is required for maintaining the balance between quiescence induction and amplification / differentiation in the postnatal MP pool. Conclusions: The tumor suppressor Nf1 maintains the myogenic progenitor (MP) pool during postnatal muscle development. Overall design: mRNA profiles of FACS sorted juvenile satellite cells isolated from 7-day-old Control (Nf1flox/+ Myf5Cre+) and Nf1 KO (Nf1flox/flox Myf5Cre+) mice.

研究目的：探究神经纤维瘤病1型基因（Nf1）调控幼年骨骼肌卫星细胞静息诱导的分子通路。方法：本研究对从出生后第7天的对照组（Nf1flox/+ Myf5Cre+）与敲除组（KO，Nf1flox/flox Myf5Cre+）小鼠后肢骨骼肌中通过荧光激活细胞分选（FACS）分离得到的幼年卫星细胞的总mRNA进行了测序。结果：本研究表明，Nf1是维持出生后肌源性祖细胞（myogenic progenitor, MP）池静息诱导与扩增/分化之间动态平衡的必需因子。结论：抑癌基因Nf1在出生后肌肉发育过程中维持肌源性祖细胞池的稳态。整体设计：对从7日龄对照组（Nf1flox/+ Myf5Cre+）与Nf1敲除组（Nf1flox/flox Myf5Cre+）小鼠中分离并经荧光激活细胞分选（FACS）得到的幼年卫星细胞进行mRNA表达谱分析。