Gene expression signatures for human interleukin-6 (IL-6) treatment in human multiple myeloma (MM) cell lines.

To investigate the effect of IL-6 on gene expression in IL-6-dependent MM cell lines, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to be associated with the cell proliferation of MM cells. ANBL-6 and FLAM-76 cells were incubated for 48 h in the absense of IL-6, and then the cells were treated with IL-6 (10 ng/mL) for 0, 6, 12, and 24 h in vitro. ANBL-6 and FLAM-76 cells were incubated for 48 h in the absense of IL-6, and then the cells were treated with IL-6 (10 ng/mL) for 0, 6, 12, and 24 h in vitro.

为探究白细胞介素6（IL-6）对IL-6依赖性多发性骨髓瘤（Multiple Myeloma, MM）细胞系的基因表达影响，本研究采用全基因组微阵列表达谱作为发现平台，以筛选潜在与MM细胞增殖相关的基因。将ANBL-6与FLAM-76细胞在不含IL-6的条件下孵育48小时，随后于体外用10 ng/mL的IL-6分别处理0、6、12及24小时。将ANBL-6与FLAM-76细胞在不含IL-6的条件下孵育48小时，随后于体外用10 ng/mL的IL-6分别处理0、6、12及24小时。