Supplementary Material for: The Expression of MAS1, an Angiotensin (1–7) Receptor, in the Eutopic Proliferative Endometria of Endometriosis Patients

<b><i>Background/Aim:</i></b> We demonstrated that AT1 and AT2 are expressed and both pathways balance the renin-angiotensin system in endometriosis. MAS1, a specific receptor of angiotensin (1–7), opposes AT1 pathway-associated tissue remodelling. It is not known whether MAS1 has an effect on the pathogenesis of endometriosis or not. <b><i>Materials and Methods:</i></b> Ovarian endometriotic tissues (endo-Ov) and eutopic endometrial tissues (endo-Em) were obtained from 29 patients with endometrial cysts. Normal endometrial tissues (cont-Em) were obtained from patients without endometriosis. Immunohistochemical staining was performed for MAS1, AT1 and AT2 in the endometriosis-associated tissues. The mRNA levels of these receptors were examined by quantitative reverse transcription PCR. <b><i>Results:</i></b> MAS1 was immune-positive at the apical side of the glandular epithelium in the endometriotic lesions. The MAS1 mRNA levels in endo-Ov were increased significantly, irrespective of the menstrual cycle phase. The MAS1 mRNA levels were significantly higher in the proliferative-tissues of the endometriosis patients than in those of the controls. The ratio of the MAS1 to the AT1 mRNA in the proliferative tissues was increased predominantly in the endometriosis patients compared with that in the controls. <b><i>Conclusion:</i></b> High MAS1 expression in the endometrium might promote the initiation of endometriosis via migration of proliferative tissue.

**背景与目的**：我们已证实AT1与AT2均有表达，且二者通路在子宫内膜异位症中对肾素-血管紧张素系统发挥平衡调控作用。MAS1作为血管紧张素(1–7)的特异性受体，可拮抗AT1通路介导的组织重构。目前尚未明确MAS1是否会对子宫内膜异位症的发病机制产生影响。
**材料与方法**：本研究从29例子宫内膜囊肿患者体内采集卵巢子宫内膜异位组织（endo-Ov）及在位子宫内膜组织（endo-Em）；从无子宫内膜异位症的受试者体内获取正常子宫内膜组织（cont-Em）。采用免疫组化染色（immunohistochemical staining）法对上述子宫内膜异位症相关组织中的MAS1、AT1及AT2进行检测；通过定量逆转录PCR（quantitative reverse transcription PCR）检测上述受体的mRNA表达水平。
**结果**：MAS1在子宫内膜异位病灶的腺上皮顶端呈现免疫阳性表达。无论所处月经周期时相如何，卵巢子宫内膜异位组织中的MAS1 mRNA水平均显著升高。子宫内膜异位症患者增殖期组织的MAS1 mRNA水平显著高于对照组。与对照组相比，子宫内膜异位症患者增殖期组织中MAS1与AT1的mRNA比值显著升高。
**结论**：子宫内膜组织中高表达的MAS1或可通过增殖组织的迁移过程，促进子宫内膜异位症的发生。