Microbiome Disruption in Oak Trees Along an Urbanization Gradient

This study investigated how urbanization disrupts the microbiomes associated with oak (genus Quercus) trees. We sampled microbial communities from four tree-associated compartments (leaves, roots, upper soils (0-15 cm), and lower soils (15-30 cm)) across an urban-to-rural gradient in Massachusetts and characterized them using 16S rRNA gene and ITS region amplicon sequencing.

本研究探究了城市化如何干扰栎属（Quercus）树木相关的微生物组。我们沿美国马萨诸塞州的城乡梯度，从四类与树木关联的微生境（叶片、根系、上层土壤（0~15 cm）及下层土壤（15~30 cm））中采集微生物群落样本，并通过16S核糖体RNA基因（16S rRNA gene）与内转录间隔区（ITS）扩增子测序技术对群落特征进行表征。