Transcription start site analysis of NIPBL depletion in cancer cell line

Transciption start site expression was studied after knockdown of NIPBL cancer cell lines (A549, H358, H2228, 22RV1, VCAP, C106, SW620, HCT116, CAMA-1) CAGE-seq was performed on shLuc and shNIPBL_#2 knockdown cells

本研究以NIPBL基因敲低的癌细胞系（A549、H358、H2228、22RV1、VCAP、C106、SW620、HCT116、CAMA-1）为对象，分析其转录起始位点（Transcription Start Site）的表达水平；实验中对转染靶向荧光素酶对照短发卡RNA（shLuc）以及靶向NIPBL的2号短发卡RNA（shNIPBL_#2）的敲低细胞系开展了CAGE-seq测序。