agronomy-2762935 data.zip

Flowers and pod abscission significantly reduces soybean yield. This study aims to identify the main signaling pathways and key candidate genes in soybean leaves that affect flower and pod abscission under shade conditions. This information will be useful for the localization and cloning of genes related to abscission. Two soybean cultivars with different abscission rates (Liaodou 32 and Shennongdou 28) were used in this experiment. The soybean leaves were subjected to 50% shading treatment and the transcriptome and metabolome was sequenced during the light-sensitive period. The effects of weak light at different growth stages on the metabolic pathways of soybean leaves and organ abscission were investigated by analyzing plant phenotype and physiological changes. The results showed that both two cultivars triggered the same molecular mechanism and similar metabolite accumulation mode by shading, but the regulations of the two cultivars were different.

花与荚果脱落（flower and pod abscission）会显著降低大豆产量。本研究旨在明确遮阴条件下，大豆叶片中影响花与荚果脱落的主要信号通路与关键候选基因，相关成果可为脱落相关基因的定位与克隆提供重要支撑。本实验选用两个脱落率存在差异的大豆品种：辽豆32（Liaodou 32）与沈农农28（Shennongdou 28），于光敏感时期对其叶片开展50%遮阴处理，并进行转录组（transcriptome）与代谢组（metabolome）测序。通过分析植株表型与生理变化，探究不同生育期弱光对大豆叶片代谢通路及器官脱落的调控效应。研究结果显示，两个供试品种在遮阴处理下均激活了相同的分子机制与相似的代谢物积累模式，但二者的调控模式存在显著差异。