DataSheet_1_Identification of a major QTL underlying sugar content in peanut kernels based on the RIL mapping population.docx

High sugar content in peanut seeds is one of the major breeding objectives for peanut flavor improvement. In order to explore the genetic control of sugar accumulation in peanut kernels, we constructed a recombinant inbred line population of 256 F2:6-7 lines derived from the Luhua11 × 06B16 cross. A high-resolution genetic map was constructed with 3692 bin markers through whole genome re-sequencing. The total map distance was 981.65 cM and the average bin marker distance was 0.27cM. A major stable QTL region (qSCB09/qSSCB09) was identified on linkage group (LG) B09 associated with both sucrose content (SC) and soluble sugar content (SSC) explaining 21.51-33.58% phenotypic variations. This major QTL region was consistently detected in three environments and mapped within a physical interval of 1.56 Mb on chromosome B09, and six candidate genes were identified. These results provide valuable information for further map-based cloning of favorable allele for sugar content in peanut.

花生种子高糖含量是改良花生风味的核心育种目标之一。为解析花生籽仁糖积累的遗传调控机制，本研究以鲁花11（Luhua11）与06B16为亲本构建杂交组合，进而获得包含256个F₂:₆₋₇代株系的重组自交系（recombinant inbred line, RIL）群体。通过全基因组重测序（whole genome re-sequencing）技术，构建了包含3692个bin标记（bin marker）的高分辨率遗传连锁图谱，该图谱总遗传距离为981.65 cM，平均bin标记间距为0.27 cM。在B09连锁群（linkage group, LG）上鉴定到一个同时与蔗糖含量（sucrose content, SC）和可溶性糖含量（soluble sugar content, SSC）相关的主效稳定数量性状位点（Quantitative Trait Locus, QTL）区域qSCB09/qSSCB09，该位点可解释21.51%~33.58%的表型变异。该主效QTL区域在三个环境中均能稳定检测到，物理定位在B09染色体1.56 Mb的区间内，同时鉴定到6个候选基因。本研究结果为后续基于图谱克隆花生糖含量相关优良等位基因提供了重要参考依据。