Effect of hpyAVIBM, a C5 cytosine methyltransferase deletion on the Helicobacter pylori transcriptome. Effect of hpyAVIBM, a C5 cytosine methyltransferase deletion on the Helicobacter pylori transcriptome

Helicobacter pylori genome is rich in restriction - modification (R-M) systems. Around 4 % of the genome codes for components of R-M systems. hpyAVIBM, which codes for a putative phase-variable C5 - cytosine methyltransferase (MTase) from H. pylori lacks a cognate restriction enzyme. Overall design: To analysis the effect of deleting hpyAVIBM on the Helicobacter pylori transcriptome, microarray analysis was done with the wild type strains and corresponding hpyAVIBM deletion strains.

幽门螺杆菌（Helicobacter pylori）基因组富含限制性修饰（R-M）系统，其基因组中约4%的序列编码该系统的组成元件。hpyAVIBM基因编码来自幽门螺杆菌的推定相变量C5胞嘧啶甲基转移酶（MTase），但该基因缺乏同源限制性内切酶。实验整体设计：为探究敲除hpyAVIBM基因对幽门螺杆菌转录组的影响，研究人员以野生型菌株及对应的hpyAVIBM缺失菌株为材料开展了微阵列分析。