Draft genome sequence of Cryptendoxyla hypophloia MUT 1750 (Ascomycota, Cephalothecaceae).

The species in the Cephalothecaceae family (Sordariomycetes) are saprobic or epiphytic, often grow on rotten wood or dead plants, and cause mycotic infection in humans. The genus Cryptendoxyla is included in the family Cephalothecaceae and can be identified by cephalothecoid peridium, cylindrical brown ascospores and a Chalara conidial stage.The strain MUT 1750 was assigned to Cryptendoxyla hypophloia by means of morphological traits and DNA sequence analysis. MUT 1750 is resistant to cycloheximide, an inhibitor of eukaryotic protein synthesis. The strain is deposited at Mycotheca Universitatis Taurinensis (MUT, www.mut.unito.it) of the Department of Life Sciences and Systems Biology, University of Turin, Turin (Italy).Here, we report the draft genome sequence of MUT 1750 obtained with next-generation sequencing (NGS) techniques for understanding the molecular basis that can lead to resistance to cycloheximide and other features of biotechnological value. Our ongoing efforts include comparing the genome of MUT 1750 with other newly sequenced Cephalothecaceae genomes, highlighting both shared and unique features. The NGS and bioinformatics project was supported by the INAIL-DIT, Research Area (PAR 2019-2021).

粪壳菌纲（Sordariomycetes）头囊壳科（Cephalothecaceae）的物种多为腐生或附生真菌，常生长于腐木或枯死植物体上，亦可引发人类真菌性感染。隐囊壳属（Cryptendoxyla）隶属于头囊壳科，可通过头囊壳型包被（cephalothecoid peridium）、圆柱形棕色子囊孢子以及Chalara无性型（Chalara conidial stage）进行鉴定。菌株MUT 1750经形态学特征与DNA序列分析，被鉴定为隐皮隐囊壳（Cryptendoxyla hypophloia）。该菌株对环己酰亚胺（cycloheximide）——一种真核蛋白质合成抑制剂——具有抗性。菌株保藏于意大利都灵大学生命与系统生物学系的都灵大学真菌保藏中心（Mycotheca Universitatis Taurinensis，缩写MUT，网址：www.mut.unito.it）。本研究报道了通过下一代测序（next-generation sequencing，NGS）技术获得的MUT 1750草图基因组序列，旨在解析其对环己酰亚胺产生抗性的分子基础，以及其他具备生物技术价值的特征。我们当前正在开展的研究工作包括将MUT 1750的基因组与其他新近测序的头囊壳科真菌基因组进行比对，以明确二者共有的及特有的基因组特征。本NGS与生物信息学研究项目得到了意大利国家工伤事故保险局-创新与技术发展部（INAIL-DIT）2019-2021研究区域项目（PAR 2019-2021）的资助。