Analyzed smFISH Dataset
收藏Figshare2020-09-01 更新2026-04-28 收录
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This datatable contains all excitatory cells (slc17a7+) across anterior, intermediate, and posterior sections of the basolateral amygdala. Metadata includes:- gene: denotes gene expressed within given cell- quant: quantified expression of a given gene, normalized so that quantification of each gene adds to 1 for a given cell- X & Y: cell position in space- position: denotes which section the cells came from (anterior intermediate, or posterior)- tsne1 & tsne2: plot these to get tsne visualization (perplexity = 35, max iteration = 6350)- cluster: cells labelled cluster 1-6 - according to hierarchical clustering (distance = Euclidean, method = Ward D2)
本数据表收录了基底外侧杏仁核(basolateral amygdala)前、中、后三个亚区中所有slc17a7阳性的兴奋性细胞。其配套元数据包含以下内容:
- 基因(gene):指代指定细胞内表达的基因
- 量化值(quant):指定基因的量化表达水平,经标准化处理后,单个细胞内所有基因的量化值之和为1
- X与Y坐标:细胞的空间位置坐标
- 分区(position):标注细胞所属的组织亚区(前部、中部或后部)
- t-SNE维度1(tsne1)与t-SNE维度2(tsne2):绘制这两个维度即可生成t分布邻域嵌入(t-SNE)可视化结果,其中困惑度(perplexity)设置为35,最大迭代次数为6350
- 聚类标签(cluster):基于层次聚类(hierarchical clustering)结果将细胞划分为1至6共6个聚类,该聚类以欧氏距离(Euclidean)作为距离度量标准,聚类方法为Ward D2
创建时间:
2020-09-01



