16s rRNA from maize rhizosphere soil bacterial

In order to study the influence of γ-PGA on bacterial community diversity under drought stress, the relative abundance and diversity of maize rhizospheric soil bacteria were analyzed by high-throughput sequencing of 16S rRNA.Amplification and High-throughput sequencing of 16s rRNA from maize rhizosphere soil bacterial were performed as described by Wang et al.(Wang et al.). The primers of V4 region of bacterial 16S rRNA were 338F (5’-ACTCCTACGGGAGGCAGCA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). High-throughput sequencing was performed by Illumina Hiseq 2000 (Illumina Inc., San Diego, USA).

为探究γ-聚谷氨酸（γ-PGA）在干旱胁迫下对细菌群落多样性的影响，本研究通过对16S核糖体RNA（16S rRNA）进行高通量测序，分析了玉米根际土壤细菌的相对丰度与群落多样性。本研究按照Wang等人（Wang et al.）的方法，对玉米根际土壤细菌的16S rRNA进行扩增及高通量测序。细菌16S rRNA的V4区扩增引物为338F（5’-ACTCCTACGGGAGGCAGCA-3’）与806R（5’-GGACTACHVGGGTWTCTAAT-3’）。高通量测序由美国加利福尼亚州圣地亚哥的Illumina公司Illumina Hiseq 2000测序平台完成。