Pprgenome.fa

Populus pruinosa, owing high tolerant ability to arid, saline and alkaline desert, is an attractive model for studying ecological adaptation and speciation in extreme environments. Here, a chromosome-level reference genome of P. pruinosa (v2.0) with 521.09 Mb and a contig N50 of 21.06 Mb by combining PacBio sequencing with Hi-C technologies were reported. Our aim was to (i) build a chromosome-level reference genome; and (ii) investigate the population structure and geographic distribution model of P. pruinosa. Our study would facilitate future genomics-assisted genetic improvement and conservation efforts for the endangered species.

灰杨（Populus pruinosa）对干旱、盐碱荒漠具有极强的耐受能力，是研究极端环境下生态适应与物种形成的理想模式物种。本研究结合PacBio测序与Hi-C技术，构建了灰杨v2.0版本的染色体级参考基因组，组装总长度达521.09 Mb，重叠群N50（contig N50）为21.06 Mb。本研究的目标主要有二：一是构建染色体级参考基因组；二是解析灰杨的种群结构与地理分布模式。本研究将为这一濒危物种的基因组辅助遗传改良与保护工作提供重要支撑。