Dynamic expression of smRNA from fecal exosome in disease progression of an inflammatory bowel disorder mouse model. Dynamic expression of smRNA from fecal exosome in disease progression of an inflammatory bowel disorder mouse model

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract affecting over 3 million adults in the United States. Despite being widespread, reliable early diagnostic tests are not available. In order to remedy this, we examined exosomal small RNA (smRNA), specifically targeting microRNA (miRNA) and piRNA from the stool samples of IBD model mice, interleukin 10 knockout mice (IL-10 KO), as a potential diagnostic marker. Stool samples were specifically chosen because they are readily available and collection is noninvasive. Overall design: Total 17 samples were analyzed; 7 and 10 replicates each for end-point and start-point samples, respectively.

炎症性肠病（Inflammatory bowel disease, IBD）是一类胃肠道慢性炎症性疾病，在美国影响超300万成年人群。尽管该病流行广泛，但目前尚无可靠的早期诊断检测手段。为解决这一临床痛点，本研究以炎症性肠病模型小鼠（白细胞介素10基因敲除小鼠，IL-10 KO）的粪便样本为研究材料，对其中的外泌体小RNA（exosomal small RNA, smRNA）进行检测，重点关注微小RNA（microRNA, miRNA）与Piwi互作RNA（piwi-interacting RNA, piRNA），以期将其作为潜在的诊断标志物。选取粪便样本的原因在于其易于获取且采集过程无创。实验整体设计：共分析17份样本，终末样本组与起始样本组分别设置7组和10组重复样本。