Analysis of the nitrate-responsive transcriptome during root nodule symbiosis in wild type, the Ljnin mutants, the nrsym1 mutants, the nrsym2 mutants, and the nrsym1 nrsym2 double mutants of Lotus japonicus. Lotus japonicus strain:Miyakojima MG-20

To gain insights into the roles of NRSYM1 and NRSYM2 in the nitrate-responsive gene expression in Lotus japonicus, we conducted RNA-seq of the roots of wild-type (MG-20), the nrsym1-1 mutants, the nrsym2 mutants, and the nrsym1-1 nrsym2 double mutants grown with or without nitrate during root nodule symbiosis. We performed RNA-seq analysis of the roots of wild-type and the Ljnin-9 mutants grown with or without rhizobia to identify LjNIN-dependent rhizobia-inducible genes.

为解析NRSYM1与NRSYM2在百脉根（Lotus japonicus）硝酸盐响应基因表达中的功能，我们对根瘤菌共生条件下添加或未添加硝酸盐的野生型（MG-20）、nrsym1-1突变体、nrsym2突变体以及nrsym1-1 nrsym2双突变体的根系开展了RNA测序（RNA-seq）。为鉴定依赖于LjNIN的根瘤菌诱导基因，我们对添加或未添加根瘤菌的野生型与Ljnin-9突变体的根系进行了RNA-seq分析。