Acipenser oxyrinchus sequencing reads

As non-teleost actinopterygians, sturgeons have not undergone a teleost-specific whole-genome duplication, however, several polyploidization events have taken place within the sturgeon family. The availability of the Atlantic sturgeon genome could offer a great opportunity to investigate the evolution of polyploid genomes, although to date there is no published reference genome for this species. Therefore, the goal of this project is to sequence and assemble the Atlantic sturgeon. For this, a juvenile female specimen originated from St. John's River (Canada) was sampled at Fischzucht Rhoenforelle (Gersfeld, Germany). Genomic DNA was extracted from red blood cells and paired-end libraries were prepared and sequenced using an Illumina HiSeq2500 instrument.

作为非真骨辐鳍鱼类（non-teleost actinopterygians），鲟鱼并未经历真骨鱼特有的全基因组复制事件（teleost-specific whole-genome duplication），但鲟科内部已发生过多次多倍化事件（polyploidization events）。尽管目前尚无大西洋鲟（Atlantic sturgeon）的公开参考基因组，但其基因组的解析将为多倍体基因组演化研究提供绝佳契机。因此，本项目的目标为对大西洋鲟进行测序与基因组组装。为此，研究人员从位于德国盖斯费尔德（Gersfeld）的Fischzucht Rhoenforelle养殖场采集了一只源自加拿大圣约翰河（St. John's River）的雌性幼体标本。研究人员从该标本的红细胞中提取基因组DNA，构建双端测序文库，并使用Illumina HiSeq2500测序仪完成测序。