The Evolving Immune Microenvironment of Human B-cell Acute Lymphoblastic Leukemia [CITE-Seq]

Single-cell RNA-seq analysis of primary human bone marrow immune populations isolated from B-ALL patient samples (matched diagnosis, remission and relapse), and healthy bone marrow donor samples. Single-cell RNA-sequencing libraries of primary human bone marrow immune populations. B-ALL patient bone marrow samples were separated into CD19+ B cell and CD19-CD45+ non-B cell fractions by flow-based sorting and then mixed at a ratio of 1:5 CD19+:CD19-CD45+ followed by scRNA-Seq.

本研究针对从匹配诊断、缓解与复发三个阶段的B细胞急性淋巴细胞白血病（B-ALL）患者样本，以及健康骨髓供体样本中分离得到的原代人骨髓免疫细胞群开展单细胞RNA测序分析。本数据集包含原代人骨髓免疫细胞群的单细胞RNA测序文库。研究人员通过流式细胞分选将B-ALL患者的骨髓样本分为CD19阳性B细胞组分与CD19阴性CD45阳性非B细胞组分，随后以1:5的比例（CD19阳性组分:CD19阴性CD45阳性组分）混合，并进行scRNA-Seq（单细胞RNA测序）。