Dietary interventions modulate global non-coding RNA expression: a role for microRNA-chromatin-mRNA crosstalk [Diet_miRNA]. Mus musculus

Using high-throughput deep sequencing, microRNA expression was profiled across six dietary intervention groups, including low fat (LF) or high fat (HF) with or without calorie restriction (LF+CR, HF+CR) or exercise (LF+Ex, HF+Ex). Overall design: Mice were treated starting at 5 weeks of age with LF or HF diet with or without CR or Ex. Liver RNA was extracted and used for small RNA-sequencing at midlife (62 weeks of age).

采用高通量深度测序（high-throughput deep sequencing）技术，对6个膳食干预组的微小RNA（microRNA）表达水平开展表达谱分析。该6个组别分别为：低脂（LF, low fat）膳食组、低脂+热量限制（LF+CR, calorie restriction）组、低脂+运动（LF+Ex, exercise）组、高脂（HF, high fat）膳食组、高脂+热量限制（HF+CR）组以及高脂+运动（HF+Ex）组。
实验整体设计如下：实验小鼠自5周龄起开始接受对应干预处理，饲喂低脂或高脂膳食，同时辅以或不辅以热量限制或运动干预。在小鼠处于中年阶段（62周龄）时，提取其肝脏RNA并进行小RNA测序（small RNA-sequencing）。